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DARC anticorps (N-Term)

Verified DARC Reactivité: Humain WB, ELISA, IHC, FACS Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN185480
  • Antigène Voir toutes DARC Anticorps
    DARC (Duffy Blood Group, Chemokine Receptor (DARC))
    Épitope
    • 12
    • 12
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 39
    • 10
    • 6
    Humain
    Hôte
    • 31
    • 8
    • 2
    • 1
    Chèvre
    Clonalité
    • 35
    • 6
    • 1
    Polyclonal
    Conjugué
    • 24
    • 4
    • 4
    • 4
    • 3
    • 3
    Cet anticorp DARC est non-conjugé
    Application
    • 28
    • 24
    • 8
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Fonction
    Duffy / FY / DARC
    Séquence
    HRAELSPSTE NSSQLDFED
    Specificité
    This antibody is expected to recognize both reported isoforms (NP_001116423.1, NP_002027.2).
     Réactivité croisée
    Humain
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1, NP_002027.2.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3

    Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Commentaires

    Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    DARC (Duffy Blood Group, Chemokine Receptor (DARC))
    Autre désignation
    ACKR1 (DARC Produits)
    Synonymes
    anticorps DARC, anticorps FY, anticorps ACKR1, anticorps CCBP1, anticorps CD234, anticorps Dfy, anticorps GPD, anticorps GpFy, anticorps WBCQ1, anticorps AA162249, anticorps Ackr1, anticorps ESTM35, anticorps Duffy blood group, chemokine receptor, anticorps atypical chemokine receptor 1 (Duffy blood group), anticorps atypical chemokine receptor 1, anticorps DARC, anticorps ACKR1, anticorps Ackr1
    Sujet
    FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D, Dfy, GpFy, WBCQ1, Fy glycoprotein, OTTHUMP00000035293, OTTHUMP00000035294
    ID gène
    2532
    NCBI Accession
    NP_001116423, NP_002027
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