GLI2 anticorps (Internal Region)

N° du produit ABIN185585

Détail du produit anti-GLI2 anticorps

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI2 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC)

Fonction: GLI2

Séquence: DLKEDLDRDD CKQE

Specificité: This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)

Homologie: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2.

Isotype: IgG

Information d'application

Indications d'application:

DS WB Results: Preliminary experiments gave an approx 55 kDa band in Human Bone Marrow and Ovary lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168 kDa according to NP_005261.2. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.

DS IHC Results: In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GLI2

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Autre désignation: GLI2 (GLI2 Produits)

Synonymes: anticorps HPE9, anticorps THP1, anticorps THP2, anticorps GLI2/GLI4, anticorps GLI3, anticorps AW546128, anticorps GLI family zinc finger 2, anticorps GLI-Kruppel family member GLI2, anticorps GLI2, anticorps Gli2

Sujet: GLI2, GLI-Kruppel family member GLI2 , THP2, oncogene GLI2, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, zinc finger protein GLI2

ID gène: 2736

NCBI Accession: NP_005261

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis