AIRE anticorps (Internal Region)

N° du produit ABIN185643

Détail du produit anti-AIRE anticorps

Antigène: AIRE (Autoimmune Regulator (AIRE))

Épitope: Internal Region

Reactivité: Humain, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIRE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Fonction: AIRE

Séquence: KDVDLSQPRK GRKP

Réactivité croisée: Boeuf (Vache), Chien, Humain, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Thymus shows nuclear staining in select thymocytes. This antibody has been successfully used in IHC on Human: Ströbel P et al. (2014) PMID: 24236644. This antibody has been successfully used in IHC on Pig:

Western Blot: Appr. 60 kDa in transfected HEK293 lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 0.5-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-AIRE (ABIN185643)

Suzuki, Suzuki, Nakai, Sembon, Fuchimoto, Onishi: "Transcriptional and histological analyses of the thymic developmental process in the fetal pig." dans: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 63, Issue 2, pp. 215-25, (2014) (PubMed).

Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." dans: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).

Détails sur AIRE

Antigène: AIRE (Autoimmune Regulator (AIRE))

Autre désignation: AIRE (AIRE Produits)

Synonymes: anticorps AIRE1, anticorps APECED, anticorps APS1, anticorps APSI, anticorps PGA1, anticorps autoimmune regulator, anticorps autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), anticorps AaeL_AAEL013794, anticorps CpipJ_CPIJ001208, anticorps CpipJ_CPIJ001209, anticorps AIRE, anticorps Aire

Sujet: AIRE, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) , AIRE1, APECED, APS1, APSI, PGA1 , autoimmune regulator (APECED protein), autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy),

ID gène: 326

NCBI Accession: NP_000374

Pathways: Chromatin Binding