Hsc70 anticorps (Internal Region)

N° du produit ABIN185659

Détail du produit anti-Hsc70 anticorps

Antigène: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Hsc70 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Fonction: HSPA8 (Isoform 1)

Séquence: EKLQGKINDE DKQK

Specificité: This antibody is expected to recognise isoform 1 (NP_006588.1) only.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Hsc70 (ABIN185659)

Johnson, Kaplitt: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." dans: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).

Détails sur Hsc70

Antigène: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Autre désignation: HSPA8 (HSPA8 Produits)

Synonymes: anticorps hsc54, anticorps hsc70, anticorps hsc71, anticorps hsp71, anticorps hsp73, anticorps hspa10, anticorps lap1, anticorps nip71, anticorps HSC54, anticorps HSC70, anticorps HSC71, anticorps HSP71, anticorps HSP73, anticorps HSPA10, anticorps LAP1, anticorps NIP71, anticorps Hsc70, anticorps 2410008N15Rik, anticorps Hsc71, anticorps Hsc73, anticorps Hsp73, anticorps Hspa10, anticorps wu:fb01g06, anticorps wu:fi48b06, anticorps heat shock protein family A (Hsp70) member 8 L homeolog, anticorps heat shock protein family A (Hsp70) member 8, anticorps heat shock 70kDa protein 8, anticorps heat shock protein 8, anticorps hspa8.L, anticorps HSPA8, anticorps Hspa8, anticorps hspa8

Sujet: HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh

ID gène: 3312, 15481, 24468

NCBI Accession: NP_006588