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SUN5 anticorps

SUN5 Reactivité: Humain, Souris, Rat WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1876373
  • Antigène Voir toutes SUN5 Anticorps
    SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))
    Reactivité
    Humain, Souris, Rat
    Hôte
    • 26
    • 2
    Lapin
    Clonalité
    • 28
    Polyclonal
    Conjugué
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp SUN5 est non-conjugé
    Application
    • 20
    • 13
    • 13
    • 4
    • 3
    • 3
    • 1
    Western Blotting (WB)
    Attributs du produit
    Expected molecular weight of target: 379 kDa
    Purification
    Affinity purification
    Isotype
    IgG
    Top Product
    Discover our top product SUN5 Anticorps primaire
  • Indications d'application
    WB 1:500-1:2000
    Restrictions
    For Research Use only
  • Validation #102344 (Immunocytochemistry)
    'Independent Validation' signe
    by
    Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Developmental Biology, Georg-August-University Göttingen
    No.
    #102344
    Date
    19.12.2017
    Antigène
    SUN5
    Numéro du lot
    Application validée
    Immunocytochemistry
    Contrôle positif
    NIH/3T3 mouse embryonic fibroblast cells overexpressing HA- and myc-tagged SPAG4L-2/SUN5
    Contrôle négative
    NIH/3T3 mouse embryonic fibroblast cells overexpressing HA- and myc-tagged SPAG4
    Conclusion

    Passed. ABIN1876373 is specific for SPAG4L-2/SUN5 and did not detect SPAG4 when ectopically expressed. Unspecific binding to other cellular proteins is nearly absent.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN1876373
    Anticorps secondaire
    goat anti-rabbit IgG DyLight488 (Thermo Scientific, 35553, lot KA130088)
    Full Protocol
    • NIH/3T3 cells (ATCC, CRL-1658) are grown in DMEM+GlutaMAX (Gibco, 31966-021, Lot 1852045) supplemented with fetal bovine serum (Gibco 270-106) and Pen/Strep (Gibco 15140), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with HA/myc-tagged SPAG4 expression plasmid or HA/myc-tagged SPAG4L-2/SUN5 (Frohnert et al., 2011) expression plasmid using EndofectinMax (GeneCopoeia) following the manufacturer's instructions.
    • Grow cells for 24h.
    • Fix cells in 3.7% paraformaldehyde (in PBS) for 15min at 4°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at room temperature.
    • Incubate cells with primary
      • rabbit anti-SUN5 antibody (antibodies-online, ABIN1876373) diluted 1:100 in PBS ON at 4° and
      • mouse anti-myc tag antibody clone (9E10) diluted 1:100 in PBS ON at 4°C.
    • Wash cells with TBST (50mM Tris-HCl, pH 7.4, 150mM NaCl, 0.1% Tween 20) for 15min at RT.
    • Incubate cells with secondary
      • goat anti-rabbit IgG DyLight488 (Thermo Scientific, 35553, lot KA130088) diluted 1:1000 in PBS for 1h at 37°C and
      • goat anti-mouse IgG (H+L) Alexa Fluor 555 (Invitrogen, A21422, lot 948498) diluted 1:1000 in PBS for 1h at 37°C.
    • Counterstain DNA with DAPI (4,6-Diamidino-2-phenylindole; Sigma D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Notes
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C.
  • Antigène
    SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))
    Autre désignation
    SPAG4L (SUN5 Produits)
    Synonymes
    anticorps SPAG4L, anticorps TSARG4, anticorps dJ726C3.1, anticorps 1700021O15Rik, anticorps SPAG4L-2, anticorps SRG4, anticorps Spag4l, anticorps Sad1 and UNC84 domain containing 5, anticorps SUN5, anticorps Sun5
    ID gène
    140732
    UniProt
    Q8TC36
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