This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This Mouse Irf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of mouse Irf3.
IRF3
Reactivité: Humain
WB, ELISA, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Marichal, Bedoret, Mesnil, Pichavant, Goriely, Trottein, Cataldo, Goldman, Lekeux, Bureau, Desmet: "Interferon response factor 3 is essential for house dust mite-induced airway allergy." dans: The Journal of allergy and clinical immunology, Vol. 126, Issue 4, pp. 836-844.e13, (2010) (PubMed).
Menachery, Pasieka, Leib: "Interferon regulatory factor 3-dependent pathways are critical for control of herpes simplex virus type 1 central nervous system infection." dans: Journal of virology, Vol. 84, Issue 19, pp. 9685-94, (2010) (PubMed).
Carrigan, Junkins, Yang, Macneil, Richardson, Johnston, Lin: "IFN regulatory factor 3 contributes to the host response during Pseudomonas aeruginosa lung infection in mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 6, pp. 3602-9, (2010) (PubMed).
Wang, Basagoudanavar, Wang, Hopewell, Albrecht, García-Sastre, Balachandran, Beg: "NF-kappa B RelA subunit is crucial for early IFN-beta expression and resistance to RNA virus replication." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 3, pp. 1720-9, (2010) (PubMed).
Farlik, Reutterer, Schindler, Greten, Vogl, Müller, Decker: "Nonconventional initiation complex assembly by STAT and NF-kappaB transcription factors regulates nitric oxide synthase expression." dans: Immunity, Vol. 33, Issue 1, pp. 25-34, (2010) (PubMed).
Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity).