This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S140 of human p27Kip1.
CDKN1B
Reactivité: Humain
WB, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
DB: 1:500
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Kajihara, Fukushige, Shioda, Tanabe, Fukunaga, Inui: "CaMKII phosphorylates serine 10 of p27 and confers apoptosis resistance to HeLa cells." dans: Biochemical and biophysical research communications, Vol. 401, Issue 3, pp. 350-5, (2010) (PubMed).
Kedde, van Kouwenhove, Zwart, Oude Vrielink, Elkon, Agami: "A Pumilio-induced RNA structure switch in p27-3' UTR controls miR-221 and miR-222 accessibility." dans: Nature cell biology, Vol. 12, Issue 10, pp. 1014-20, (2010) (PubMed).
Canbay, Eraltan, Cercel, Isbir, Gazioglu, Aydogan, Cacina, Cengiz, Ferahman, Zengin, Unal: "CCND1 and CDKN1B polymorphisms and risk of breast cancer." dans: Anticancer research, Vol. 30, Issue 7, pp. 3093-8, (2010) (PubMed).
Do Nascimento Borges, Burbano, Harada: "Absence of CIP1/KIP1 hypermethylation in gastric cancer patients from Northern Brazil." dans: In vivo (Athens, Greece), Vol. 24, Issue 4, pp. 579-82, (2010) (PubMed).
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq].