This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This rat PARP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S373 of rat PARP1.
PARP1
Reactivité: Humain
WB, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
DB: 1:500
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Beneke, Scherr, Ponath, Popp, Bürkle: "Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span." dans: Mechanisms of ageing and development, Vol. 131, Issue 5, pp. 366-9, (2010) (PubMed).
Kondo, Obitsu, Ohta, Matsunami, Otsuka, Teshima: "Poly(ADP-ribose) polymerase (PARP)-1-independent apoptosis-inducing factor (AIF) release and cell death are induced by eleostearic acid and blocked by alpha-tocopherol and MEK inhibition." dans: The Journal of biological chemistry, Vol. 285, Issue 17, pp. 13079-91, (2010) (PubMed).
Zaalishvili, Margiani, Kutalia, Suladze, Zaalishvili: "Automodification of PARP-1 mediates its tight binding to the nuclear matrix." dans: Biochemical and biophysical research communications, Vol. 393, Issue 1, pp. 123-5, (2010) (PubMed).
Drel, Xu, Zhang, Pavlov, Shevalye, Slusher, Obrosova: "Poly(Adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy." dans: Endocrinology, Vol. 150, Issue 12, pp. 5273-83, (2009) (PubMed).
Adamczyk, Kaźmierczak: "Alpha-synuclein inhibits poly (ADP-ribose) polymerase-1 (PARP-1) activity via NO-dependent pathway." dans: Folia neuropathologica / Association of Polish Neuropathologists and Medical Research Centre, Polish Academy of Sciences, Vol. 47, Issue 3, pp. 247-51, (2009) (PubMed).