This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This USP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-383 amino acids from the C-terminal region of human USP14.
USP14
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Hyrskyluoto, Bruelle, Lundh, Do, Kivinen, Rappou, Reijonen, Waltimo, Petersén, Lindholm, Korhonen: "Ubiquitin-specific protease-14 reduces cellular aggregates and protects against mutant huntingtin-induced cell degeneration: involvement of the proteasome and ER stress-activated kinase IRE1?." dans: Human molecular genetics, Vol. 23, Issue 22, pp. 5928-39, (2014) (PubMed).
Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." dans: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, (2010) (PubMed).
Chen, Qin, Li, Walters, Wilson, Mei, Wilson: "The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 35, pp. 10909-19, (2009) (PubMed).
Nagai, Kadowaki, Maruyama, Takeda, Nishitoh, Ichijo: "USP14 inhibits ER-associated degradation via interaction with IRE1alpha." dans: Biochemical and biophysical research communications, Vol. 379, Issue 4, pp. 995-1000, (2009) (PubMed).
Mines, Goodwin, Limbird, Cui, Fan: "Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation." dans: The Journal of biological chemistry, Vol. 284, Issue 9, pp. 5742-52, (2009) (PubMed).
Koulich, Li, DeMartino: "Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome." dans: Molecular biology of the cell, Vol. 19, Issue 3, pp. 1072-82, (2008) (PubMed).
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.