Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogène
Purified His-tagged TBP protein was used to produced this monoclonal antibody.
TBP
Reactivité: Humain
ELISA, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000. IHC-P: 1:25
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Hoffman, Sinn, Yamamoto, Wang, Roy, Horikoshi, Roeder: "Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID)." dans: Nature, Vol. 346, Issue 6282, pp. 387-90, (1990) (PubMed).
Peterson, Tanese, Pugh, Tjian: "Functional domains and upstream activation properties of cloned human TATA binding protein." dans: Science (New York, N.Y.), Vol. 248, Issue 4963, pp. 1625-30, (1990) (PubMed).
Kao, Lieberman, Schmidt, Zhou, Pei, Berk: "Cloning of a transcriptionally active human TATA binding factor." dans: Science (New York, N.Y.), Vol. 248, Issue 4963, pp. 1646-50, (1990) (PubMed).
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.