Nitrogenase anticorps
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- Antigène Tous les produits Nitrogenase (Nifh)
- Nitrogenase (Nifh)
- Reactivité
- Rhodopseudomonas palustris
- Hôte
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Poulet
- Clonalité
- Polyclonal
- Application
- Western Blotting (WB)
- Réactivité croisée (Details)
- No cross-reactivity with: Synechococcus sp. PCC 7942 and Synechocystis
- Homologie
- Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Frankia sp. (strain EAN1pec), Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp , Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, euryachaeotes, Azotobacter vinelandii (Gram-), Klebsiella pneumonia, cyanobacteria, Enterobacter genera, able to fix atmoshperic nitrogen
- Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 27 / 32.5 kDa
- Purification
- affinity purified
- Immunogène
- KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296
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- Indications d'application
- Recommended Dilution 1 : 2 000 (WB).
- Commentaires
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This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.36 µg/µL
- Buffer
- PBS pH 8.0+ 0.02 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock. - Stock
- 4 °C
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Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." dans: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).
: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." dans: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).
: "
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Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." dans: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).
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- Antigène
- Nitrogenase (Nifh)
- Abstract
- Nifh Produits
- Sujet
- Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.
- Poids moléculaire
- expected: 27 kDa, apparent: 32.5 kDa
- UniProt
- P26254, Q2JP78, P33178, Q51296
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