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Nitrogenase anticorps

Nifh Reactivité: Rhodopseudomonas palustris WB Hôte: Poulet Polyclonal unconjugated
N° du produit ABIN190742
  • Antigène Tous les produits Nitrogenase (Nifh)
    Nitrogenase (Nifh)
    Reactivité
    Rhodopseudomonas palustris
    Hôte
    Poulet
    Clonalité
    Polyclonal
    Application
    Western Blotting (WB)
    Réactivité croisée (Details)
    No cross-reactivity with: Synechococcus sp. PCC 7942 and Synechocystis
    Homologie
    Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Frankia sp. (strain EAN1pec), Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp , Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, euryachaeotes, Azotobacter vinelandii (Gram-), Klebsiella pneumonia, cyanobacteria, Enterobacter genera, able to fix atmoshperic nitrogen
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 27 / 32.5 kDa
    Purification
    affinity purified
    Immunogène
    KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296
  • Indications d'application
    Recommended Dilution 1 : 2 000 (WB).
    Commentaires

    This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.36 µg/µL
    Buffer
    PBS pH 8.0+ 0.02 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
    Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
    Stock
    4 °C
  • Halimatul, Ehira, Awai: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." dans: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).

    Levitan, Brown, Sudhaus, Campbell, LaRoche, Berman-Frank: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." dans: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).

  • Antigène
    Nitrogenase (Nifh)
    Abstract
    Nifh Produits
    Sujet
    Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.
    Poids moléculaire
    expected: 27 kDa, apparent: 32.5 kDa
    UniProt
    P26254, Q2JP78, P33178, Q51296
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