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FH anticorps (Internal Region)

Verified FH Reactivité: Humain WB, ELISA, IHC, EIA Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN190758
  • Antigène Voir toutes FH Anticorps
    FH (Fumarate Hydratase (FH))
    Épitope
    • 15
    • 8
    • 7
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 74
    • 18
    • 16
    • 8
    • 1
    Humain
    Hôte
    • 65
    • 17
    • 1
    Chèvre
    Clonalité
    • 66
    • 17
    Polyclonal
    Conjugué
    • 41
    • 10
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp FH est non-conjugé
    Application
    • 52
    • 33
    • 22
    • 17
    • 13
    • 13
    • 10
    • 10
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
    Fonction
    Fumarase / FH
    Séquence
    HPNDHVNKSQ SSND
    Homologie
    Human, Mouse, Rat, Dog
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.
    Isotype
    IgG
    Top Product
    Discover our top product FH Anticorps primaire
  • Indications d'application

    DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.

    DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Commentaires

    Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    FH (Fumarate Hydratase (FH))
    Autre désignation
    Fumarate Hydratase (FH Produits)
    Synonymes
    anticorps FH, anticorps DDBDRAFT_0205237, anticorps DDBDRAFT_0231397, anticorps DDBDRAFT_0231400, anticorps DDB_0205237, anticorps DDB_0231397, anticorps DDB_0231400, anticorps HLRCC, anticorps LRCC, anticorps MCL, anticorps MCUL1, anticorps Fh1, anticorps im:7152785, anticorps ns:zf-e152, anticorps zf-e152, anticorps zgc:66253, anticorps zgc:77498, anticorps Fh, anticorps Fh-1, anticorps fumarate hydratase, anticorps fumarate hydratase 1, anticorps FH, anticorps fumH, anticorps Fh, anticorps fh, anticorps Fh1
    Sujet
    Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata
    ID gène
    2271
    NCBI Accession
    NP_000134
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