CD43 anticorps (PE)

N° du produit ABIN192107

Détail du produit anti-CD43 anticorps (PE)

Antigène: CD43 (SPN) (Sialophorin (SPN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Human T lymphocytes.

Clone: MEM-59

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD43 (PE) (ABIN192107)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." dans: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." dans: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." dans: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

Alvarado, Klassen, Cerny, Horejsí, Schmidt: "MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation." dans: European journal of immunology, Vol. 25, Issue 4, pp. 1051-5, (1995) (PubMed).

Stefanová, Hilgert, Angelisová, Kristofová, Horejsí: "Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin." dans: Folia biologica, Vol. 34, Issue 4, pp. 255-65, (1989) (PubMed).

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: CD43 (SPN Produits)

Synonymes: anticorps CD43, anticorps GALGP, anticorps GPL115, anticorps LSN, anticorps A630014B01Rik, anticorps Cd43, anticorps Galgp, anticorps Ly-48, anticorps Ly48, anticorps Lsn, anticorps Lsn1, anticorps gpL115, anticorps sialophorin, anticorps SPN, anticorps Spn

Sujet: Sialophorin,CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.,Leukosialin, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP, GP5

ID gène: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity