CD48 anticorps (APC)

N° du produit ABIN192135

Détail du produit anti-CD48 anticorps (APC)

Antigène: CD48

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD48 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: The antibody MEM-102 reacts with CD48 (Blast-1), a 40-47 kDa GPI-anchored extracellular membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells, it is negative on granulocytes, platelets and erythrocytes.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Raji human Burkitt's lymphoma cell line

Clone: MEM-102

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD48 (APC) (ABIN192135)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Carlsten, Baumann, Simonsson, Jaedersten, Forsblom, Hammarstedt, Bryceson, Ljunggren, Hellstroem-Lindberg, Malmberg: "Reduced DNAM-1 expression on bone marrow NK cells associated with impaired killing of CD34(+) blasts in myelodysplastic syndrome." dans: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, (2010) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." dans: Blood, Vol. 93, Issue 4, pp. 1437-9, (1999) (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." dans: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Korínek, Stefanová, Angelisová, Hilgert, Horejsí: "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." dans: Immunogenetics, Vol. 33, Issue 2, pp. 108-12, (1991) (PubMed).

Détails sur CD48

Antigène: CD48

Autre désignation: CD48 (CD48 Produits)

Synonymes: anticorps AI449234, anticorps AW610730, anticorps BCM1, anticorps BLAST, anticorps BLAST-1, anticorps BLAST1, anticorps Bcm-1, anticorps MEM-102, anticorps SLAMF2, anticorps Sgp-60, anticorps hCD48, anticorps mCD48, anticorps CD48 antigen, anticorps Cd48 molecule, anticorps CD48 molecule, anticorps Cd48, anticorps CD48

Sujet: CD48 Molecule,CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.,BCM1, BLAST, BLAST1, SLAMF2

ID gène: 962

UniProt: P09326