PHKB
Reactivité: Humain, Souris
WB, ELISA, IHC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:500- 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C,-20 °C
Oppermann, Gnad, Olsen, Hornberger, Greff, Kéri, Mann, Daub: "Large-scale proteomics analysis of the human kinome." dans: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1751-64, (2009) (PubMed).
Beausoleil, Villén, Gerber, Rush, Gygi: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." dans: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, (2006) (PubMed).
Wüllrich-Schmoll, Kilimann: "Structure of the human gene encoding the phosphorylase kinase beta subunit (PHKB)." dans: European journal of biochemistry / FEBS, Vol. 238, Issue 2, pp. 374-80, (1996) (PubMed).
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.