Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C,-20 °C
Adachi, Sekiya, Miyachi, Matsuno, Hinoda, Imai, Yachi: "Molecular cloning of a novel protein-tyrosine phosphatase SH-PTP3 with sequence similarity to the src-homology region 2." dans: FEBS letters, Vol. 314, Issue 3, pp. 335-9, (1993) (PubMed).
Freeman, Plutzky, Neel: "Identification of a human src homology 2-containing protein-tyrosine-phosphatase: a putative homolog of Drosophila corkscrew." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11239-43, (1993) (PubMed).
Bastien, Ramachandran, Liu, Adam: "Cloning, expression and mutational analysis of SH-PTP2, human protein-tyrosine phosphatase." dans: Biochemical and biophysical research communications, Vol. 196, Issue 1, pp. 124-33, (1993) (PubMed).
Ahmad, Banville, Zhao, Fischer, Shen: "A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 6, pp. 2197-201, (1993) (PubMed).
Vogel, Lammers, Huang, Ullrich: "Activation of a phosphotyrosine phosphatase by tyrosine phosphorylation." dans: Science (New York, N.Y.), Vol. 259, Issue 5101, pp. 1611-4, (1993) (PubMed).
Antigène
PTPN11
(Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.