ERI1 anticorps

N° du produit ABIN1944959

Détail du produit anti-ERI1 anticorps

Antigène: ERI1 (Exoribonuclease 1 (ERI1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERI1 est non-conjugé

Application: Western Blotting (WB)

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C,-20 °C

Références pour anticorps anti-ERI1 (ABIN1944959)

Yang, Purdy, Marzluff, Dominski: "Characterization of 3'hExo, a 3' exonuclease specifically interacting with the 3' end of histone mRNA." dans: The Journal of biological chemistry, Vol. 281, Issue 41, pp. 30447-54, (2006) (PubMed).

Kennedy, Wang, Ruvkun: "A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans." dans: Nature, Vol. 427, Issue 6975, pp. 645-9, (2004) (PubMed).

Détails sur ERI1

Antigène: ERI1 (Exoribonuclease 1 (ERI1))

Autre désignation: ERI1 (ERI1 Produits)

Sujet: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication- dependent histone pre-mRNAs.

Poids moléculaire: 40064 Da

ID gène: 90459

UniProt: Q8IV48