The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. NMDAR1 (phospho-Ser896) antibody detects endogenous levels of NMDAR1 only when phosphorylated at serine 896.
Purification
Immunoaffinity chromatography.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from human NMDAR1 around the phosphorylation site of serine 896 (R-R-SP-S-K).
NMDA R1
Reactivité: Humain, Rat, Souris
IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Suitable for use in Western blot (1: 500approx. 1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
NMDA receptor subtypes of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. NMDAR1 plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission and is involved in the cell surface targeting of NMDA receptors.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1