L’anticorps anti-RPS6KA1 Polyclonal Lapin est utilisé pour la détection de RPS6KA1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF et IHC (p).
This antibody detects endogenous levels of p90RSK only when phosphorylated at Threonine 348.
Purification
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from mouse p90RSK around the phosphorylation site of threonine 348 (S-R-TP-P-R).
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody (in aliquots) at -20 °C.
Antigène
RPS6KA1
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))