Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
This antibody detects endogenous levels of LIMK2 only when phosphorylated at Threonine 505.
Purification
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
LIMK2
Reactivité: Humain
WB, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2