Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
This antibody detects endogenous levels of ADD1 only when phosphorylated at Serine 726.
Purification
Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-Sp-F-L).
ADD1
Reactivité: Humain
WB, FACS
Hôte: Lapin
Polyclonal
RB57511
unconjugated
Indications d'application
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers alpha/beta, and beta/gamma. The molecular weights for the various isoforms alpha=120 kD, beta=110kD, gamma=80kD. The alpha adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kinases A and C. This protein has been reported to promote the assembly of the spectrinactin network. Polymorphisms in alpha adducin are associated with cardiovascular disease and hypertension. Alpha adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, alpha adducin can regulate membrane ruffling and cell motility.Synonyms: ADDA, Erythrocyte adducin subunit alpha