RPS6 anticorps (pSer235)
Aperçu rapide pour RPS6 anticorps (pSer235) (ABIN196982)
Antigène
Voir toutes RPS6 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pSer235
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Specificité
- This antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at Serine 235.
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Purification
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogène
- The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).
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Indications d'application
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- RPS6 (Ribosomal Protein S6 (RPS6))
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Autre désignation
- RPS6
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Sujet
- Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33
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ID gène
- 6194
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NCBI Accession
- NP_001001
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UniProt
- P62753
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Pathways
- Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
Antigène
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