Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
PKCδ antibody detects endogenous levels of PKC-delta only when phosphorylated at Serine 645.
Purification
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from Human PKC-delta around the phosphorylation site of Serine 645 (R-L-Sp-Y-S).
PKCd
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
anticorps MAY1, anticorps PKCD, anticorps nPKC-delta, anticorps AI385711, anticorps D14Ertd420e, anticorps PKC[d], anticorps PKCdelta, anticorps Pkcd, anticorps fb09d10, anticorps fv43b11, anticorps prkcd, anticorps wu:fb09d10, anticorps wu:fv43b11, anticorps zgc:56558, anticorps PKC-delta, anticorps may1, anticorps npkc-delta, anticorps pkc-delta2, anticorps pkcd, anticorps tpa-1, anticorps protein kinase C delta, anticorps protein kinase C, delta, anticorps protein kinase C, delta a, anticorps protein kinase C delta L homeolog, anticorps PRKCD, anticorps Prkcd, anticorps prkcda, anticorps prkcd.L, anticorps prkcd
Sujet
Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.Synonyms: PKC delta, PKC-delta, PKCD, Protein kinase C delta type