JunD anticorps (Ser255)

N° du produit ABIN197142

Détail du produit anti-JunD anticorps

Antigène: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Épitope: Ser255

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp JunD est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: JunD antibody detects endogenous levels of total JunD protein.

Purification: Affinity-chromatography using epitope-specific immunogen

Immunogène: Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of serine 255 (G-E-Sp-P-P).

Information d'application

Indications d'application: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS( without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur JunD

Antigène: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Autre désignation: Jun-D (JUND Produits)

Synonymes: anticorps si:dkey-251j8.3, anticorps AP-1, anticorps Jund1, anticorps JunD proto-oncogene, AP-1 transcription factor subunit, anticorps jun D proto-oncogene S homeolog, anticorps jun D proto-oncogene, anticorps jund, anticorps jund.S, anticorps JUND, anticorps Jund

Sujet: JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.Synonyms: JUND, Transcription factor jun-D

ID gène: 3727

NCBI Accession: NP_005345

UniProt: P17535