This antibody detects endogenous levels of total CaMKII protein.
Purification
Affinity chromatography
Immunogène
The antiserum was produced against synthesized non-phosphopeptide derived from human CaMKII around the phosphorylation site of Threonine 286 (Q-E-Tp-V-D).
CAMK2A
Reactivité: Humain
ELISA, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody (in aliquots) at -20 °C.
Antigène
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
anticorps camk2, anticorps camk2b, anticorps zgc:112538, anticorps zgc:123320, anticorps CAMKA, anticorps CaMKII, anticorps R74975, anticorps mKIAA0968, anticorps PK2CDD, anticorps PKCCD, anticorps calcium/calmodulin dependent protein kinase II alpha, anticorps calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog, anticorps calcium/calmodulin-dependent protein kinase II alpha, anticorps CAMK2A, anticorps camk2a.S, anticorps camk2a, anticorps Camk2a
Sujet
CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968