CD3 anticorps (Biotin)

N° du produit ABIN1981855

Détail du produit anti-CD3 anticorps (Biotin)

Antigène: CD3

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificité: The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.

Réactivité croisée (Details): Mouse

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogène: Mouse BM10-37 cytotoxic T lymphocytes

Clone: 145-2C11

Isotype: IgG kappa

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-2 μg/mL.

Commentaires:

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze. Do not use after expiration date stamped on vial label.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD3 (Biotin) (ABIN1981855)

Han, Murray-Segal, Gershenzon, Zhang, Hodder, Pietersz, Mottram: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." dans: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, (2000) (PubMed).

Kearse: "Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes." dans: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14152-7, (1998) (PubMed).

Kinnaert, Pradier, Bournonville, Habrant, Goldman, Van Geertruyden et al.: "Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal ..." dans: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 9, Issue 4, pp. 386-91, (1997) (PubMed).

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." dans: Transplantation, Vol. 60, Issue 8, pp. 828-35, (1995) (PubMed).

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." dans: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, (1995) (PubMed).

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." dans: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, (1994) (PubMed).

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, (1994) (PubMed).

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, (1991) (PubMed).

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, (1987) (PubMed).

Samelson, OShea, Luong, Ross, Urdahl, Klausner, Bluestone: "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 8, pp. 2708-14, (1987) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3 (CD3 Produits)

Synonymes: anticorps CD3, anticorps AI504783, anticorps CD3epsilon, anticorps T3e, anticorps cd3-gamma, anticorps cd3g, anticorps cd3g-a, anticorps cd3g/d, anticorps cd3gd, anticorps t3g, anticorps CD3e molecule, anticorps CD3d molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps CD3g molecule S homeolog, anticorps CD3E, anticorps CD3D, anticorps Cd3e, anticorps cd3g.S

Sujet: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

ID gène: 12501

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway