CTLA4 anticorps (PE)
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- Antigène Voir toutes CTLA4 Anticorps
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CTLA4 est conjugé à/à la PE
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Application
- Flow Cytometry (FACS)
- Specificité
- The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
- Réactivité croisée (Details)
- Human
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- Human CD152-IgG heavy chain fusion protein
- Clone
- BNI3
- Isotype
- IgG2a
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- Indications d'application
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- Commentaires
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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[The use of multi-color flow cytometry for identification of functional markers of nTregs in patients with severe asthma]." dans: Pneumonologia i alergologia polska, Vol. 80, Issue 5, pp. 389-401, (2012) (PubMed).
: "Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands." dans: BMC biotechnology, Vol. 7, pp. 51, (2007) (PubMed).
: "Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis." dans: Clinical and experimental immunology, Vol. 126, Issue 1, pp. 143-50, (2001) (PubMed).
: "Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection." dans: Clinical and experimental immunology, Vol. 115, Issue 3, pp. 451-7, (1999) (PubMed).
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[The use of multi-color flow cytometry for identification of functional markers of nTregs in patients with severe asthma]." dans: Pneumonologia i alergologia polska, Vol. 80, Issue 5, pp. 389-401, (2012) (PubMed).
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- Antigène
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- Autre désignation
- CD152 (CTLA4 Produits)
- Synonymes
- anticorps CTLA-4, anticorps CTLA4, anticorps CD152, anticorps Cd152, anticorps Ctla-4, anticorps Ly-56, anticorps CD, anticorps CELIAC3, anticorps GRD4, anticorps GSE, anticorps IDDM12, anticorps sCTLA4, anticorps cytotoxic T-lymphocyte associated protein 4, anticorps cytotoxic T-lymphocyte-associated protein 4, anticorps CTLA4, anticorps Ctla4
- Sujet
- Cytotoxic T-lymphocyte associated protein 4,CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.,CTLA4, GSE, GRD4
- ID gène
- 1493
- UniProt
- P16410
- Pathways
- Cancer Immune Checkpoints
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