Perforin 1 anticorps (PE)
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- Antigène Voir toutes Perforin 1 (PRF1) Anticorps
- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
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Reactivité
- Humain, Boeuf (Vache)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Perforin 1 est conjugé à/à la PE
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Application
- Flow Cytometry (FACS)
- Specificité
- The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
- Aucune reactivité croisée
- Souris
- Réactivité croisée (Details)
- Human, Bovine
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- purified granules from human YT lymphoma cell line
- Clone
- DG9
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product PRF1 Anticorps primaire
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- Indications d'application
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
- Commentaires
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." dans: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).
: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." dans: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).
: "Flow cytometric detection of perforin upregulation in human CD8 T cells." dans: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).
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Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." dans: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).
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- Antigène
- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
- Autre désignation
- Perforin (PRF1 Produits)
- Synonymes
- anticorps FLH2, anticorps HPLH2, anticorps P1, anticorps PFN1, anticorps PFP, anticorps PRF1, anticorps Pfn, anticorps Pfp, anticorps Prf-1, anticorps Cyta, anticorps RATCYTA, anticorps LOC443187, anticorps perforin, anticorps prf1, anticorps cytolysin, anticorps perforin-1, anticorps perforin-1-like, anticorps perforin 1, anticorps perforin 1 (pore forming protein), anticorps perforin, anticorps perforin 1 L homeolog, anticorps PRF1, anticorps Prf1, anticorps LOC443187, anticorps prf1, anticorps prf1.L
- Sujet
- Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2
- ID gène
- 5551
- UniProt
- P14222
- Pathways
- Apoptose, Caspase Cascade in Apoptosis
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