FASL anticorps (PE)

N° du produit ABIN1981904

Détail du produit anti-FASL anticorps (PE)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FASL est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: L5178Y mouse T lymphoma cells expressing recombinant human CD178

Clone: NOK-1

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-FASL (PE) (ABIN1981904)

Ji, Chen, Braciale, Cloyd: "Apoptosis induced in HIV-1-exposed, resting CD4+ T cells subsequent to signaling through homing receptors is Fas/Fas ligand-mediated." dans: Journal of leukocyte biology, Vol. 81, Issue 1, pp. 297-305, (2006) (PubMed).

Jodo, Pidiyar, Xiao, Furusaki, Sharma, Koike, Ju: "Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand-mediated cytotoxicity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 8, pp. 4470-4, (2005) (PubMed).

Chopin, Barei-Moniri, Maillé, Le Frère-Belda, Muscatelli-Groux, Merendino, Lecerf, Stoppacciaro, Velotti: "Human urinary bladder transitional cell carcinomas acquire the functional Fas ligand during tumor progression." dans: The American journal of pathology, Vol. 162, Issue 4, pp. 1139-49, (2003) (PubMed).

Ghadimi, Sanzenbacher, Thiede, Wenzel, Jing, Plomann, Borkhardt, Kabelitz, Janssen: "Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178)." dans: FEBS letters, Vol. 519, Issue 1-3, pp. 50-8, (2002) (PubMed).

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." dans: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).

Détails sur FASL

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: CD178 / Fas-L (FASL Produits)

Synonymes: anticorps ALPS1B, anticorps APT1LG1, anticorps APTL, anticorps CD178, anticorps CD95-L, anticorps CD95L, anticorps FASL, anticorps TNFSF6, anticorps fasl, anticorps Fas-L, anticorps Faslg, anticorps Tnfsf6, anticorps gld, anticorps Apt1Lg1, anticorps Fasl, anticorps FASLG, anticorps zgc:162027, anticorps Fas ligand, anticorps Fas ligand L homeolog, anticorps Fas ligand (TNF superfamily, member 6), anticorps FASLG, anticorps faslg.L, anticorps Fasl, anticorps Faslg, anticorps faslg

Sujet: Fas ligand,CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.,APTL, FASL, FAS-ligand, CD95L, TNFSF6, TNLG1A

ID gène: 356

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity