GAPDH anticorps

N° du produit ABIN2017607

Détail du produit anti-GAPDH anticorps

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Porc, Boeuf (Vache), Chévre, Poisson

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: GAPDH Antibody, mAb, Mouse detects endogenous levels of human, pig, cow, goat, and fish GAPDH.

Réactivité croisée (Details): This product has been not tested with other species yet.

Attributs du produit: Fusion Partner: Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Purification: Protein A affinity column

Immunogène: Human GAPDH protein from erythrocytes

Clone: 3B1E9

Isotype: IgG2a kappa

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/mL
Western blot: 0.1-1.0 μg/mL.
Other Application: User optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: Lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Références pour anticorps anti-GAPDH (ABIN2017607)

Chen, Caporizzo, Bedi, Vite, Bogush, Robison, Heffler, Salomon, Kelly, Babu, Morley, Margulies, Prosser: "Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure." dans: Nature medicine, Vol. 24, Issue 8, pp. 1225-1233, (2019) (PubMed).

Ju, Gao, Hou, Wang, Zhang, Sun, Zhang, Xu, Shi, Hu, Zhang: "Neuroprotective effect of chondroitin sulfate on SH‑SY5Y cells overexpressing wild‑type or A53T mutant α‑synuclein." dans: Molecular medicine reports, Vol. 16, Issue 6, pp. 8721-8728, (2018) (PubMed).

Wang, Mir: "Andes virus nucleocapsid protein interrupts protein kinase R dimerization to counteract host interference in viral protein synthesis." dans: Journal of virology, Vol. 89, Issue 3, pp. 1628-39, (2015) (PubMed).

Yang, Li, Liu, He, Yuan, Li, Cao, Kim: "Mx1-cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype." dans: Genesis (New York, N.Y. : 2000), Vol. 51, Issue 3, pp. 201-9, (2013) (PubMed).

Cassel, Reitz: "Ubiquilin-2 (UBQLN2) binds with high affinity to the C-terminal region of TDP-43 and modulates TDP-43 levels in H4 cells: characterization of inhibition by nucleic acids and 4-aminoquinolines." dans: Biochimica et biophysica acta, Vol. 1834, Issue 6, pp. 964-71, (2013) (PubMed).

Martins, Collado-Romero, Martínez-Gomáriz, Carvajal, Gil, Lucena, Moreno, Garrido: "Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection." dans: Journal of proteomics, Vol. 75, Issue 14, pp. 4457-70, (2012) (PubMed).

Cassel, McDonnell, Velvadapu, Andrianov, Reitz: "Characterization of a series of 4-aminoquinolines that stimulate caspase-7 mediated cleavage of TDP-43 and inhibit its function." dans: Biochimie, Vol. 94, Issue 9, pp. 1974-81, (2012) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH (GAPDH Produits)

Synonymes: anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985

Sujet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimers and Huntingtons disease. GAPDH Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with human GAPDH protein from erythrocytes.

Poids moléculaire: Predicted Band Size: 36 KD
Observed Band Size: 36 KD