CD8 anticorps (PE-Cy5)
-
- Antigène Voir toutes CD8 Anticorps
- CD8
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp CD8 est conjugé à/à la PE-Cy5
-
Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Attributs du produit
- 4ARiv11 reacts with the 32 kDa a chain of CD8.
- Clone
- 4ARIV11
- Isotype
- IgG1
- Top Product
- Discover our top product CD8 Anticorps primaire
-
-
- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
-
- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
-
- Antigène
- CD8
- Autre désignation
- CD8 (CD8 Produits)
- Synonymes
- anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps CD8a molecule, anticorps CD8A
- Sujet
- This molecule is expressed on the T suppressor/cytotoxic cell population (which comprises about 1/3 of the peripheral blood T lymphocytes total population) and with most of thymocytes, as well as a subset of NK cells. CD8 expresses as either a heterodimer with the CD8b chain (CD8ab) or as a homodimer (CD8aa or CD8bb). CD8 acts as a co-receptor with MHC Class I restricted TCRs in antigen recognition. CD8 function is important for positive selection of MHC Class I restricted CD8+ T cells during T cell development.
-