CD57 anticorps (FITC)

N° du produit ABIN2144649

Détail du produit anti-CD57 anticorps (FITC)

Antigène: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD57 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Attributs du produit: 4AHNK-1 reacts with HNK1 Molecule, a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein. CD57 expressed on 7-35 % of normal peripheral blood lymphocytes including a subset of naturel killer cells, a subset of CD8+ peripheral blood suppressor / cytotoxic T cells, and on some neural tissues. HNK is not expression on granulocytes, platelets, red blood cells and thymocytes.

Clone: 4AHNK-1

Isotype: IgM

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD57

Antigène: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Autre désignation: CD57 (B3GAT1 Produits)

Synonymes: anticorps CD57, anticorps GLCATP, anticorps GLCUATP, anticorps HNK1, anticorps LEU7, anticorps NK-1, anticorps NK1, anticorps 0710007K08Rik, anticorps AI846286, anticorps GlcAT-P, anticorps Glcatp, anticorps Hnk1, anticorps Hnk-1, anticorps b3gat1, anticorps si:dkey-22o20.1, anticorps cd57, anticorps glcatp, anticorps glcuatp, anticorps hnk-1, anticorps hnk1, anticorps leu7, anticorps nk-1, anticorps nk1, anticorps beta-1,3-glucuronyltransferase 1, anticorps beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), anticorps beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) a, anticorps beta-1,3-glucuronyltransferase 1 L homeolog, anticorps B3GAT1, anticorps B3gat1, anticorps b3gat1a, anticorps b3gat1.L

Pathways: Glycosaminoglycan Metabolic Process