ITGB1 anticorps (PE)

N° du produit ABIN2144839

Détail du produit anti-ITGB1 anticorps (PE)

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGB1 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Attributs du produit: 4ATS2/16.2.1 reacts with 130 kDa integrin b1 , which has a broad tissue distribution . It is expressed on lympnocytes , monocytes and weakly on granulovytes, but not on erythrocytes. On T cells, CD29 is more highly expressed on memory cells than naive cells. Integrin chain b asociated with integrin a subunits 1-6 ( CD49a-f) to form CD49/CD29 heterodimers that are involved in cell-cell and cell-matrix adhesion.It has been reported that CD29 is a critical molecule for embryogenesis and development. It also essential to the differentiation of hematopoietic stem cells and associated with tumor progression and metastasis.This clone is cross reactive with non-human primate.

Clone: 4ATS2-16-2-1

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur ITGB1

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Autre désignation: CD29 (ITGB1 Produits)

Synonymes: anticorps CD29, anticorps FNRB, anticorps GPIIA, anticorps MDF2, anticorps MSK12, anticorps VLA-BETA, anticorps VLAB, anticorps 4633401G24Rik, anticorps AA409975, anticorps AA960159, anticorps ENSMUSG00000051907, anticorps Fnrb, anticorps Gm9863, anticorps gpIIa, anticorps cd29, anticorps fnrb, anticorps gpiia, anticorps itgb1, anticorps mdf2, anticorps msk12, anticorps vla-beta, anticorps vlab, anticorps itgb1-a, anticorps itgb1-b, anticorps integrin subunit beta 1, anticorps integrin beta 1 (fibronectin receptor beta), anticorps integrin subunit beta 1 S homeolog, anticorps integrin beta 1, anticorps integrin subunit beta 1 L homeolog, anticorps ITGB1, anticorps Itgb1, anticorps itgb1.S, anticorps LOC100009173, anticorps itgb1.L

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome