CD37 anticorps (PE)
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- Antigène Voir toutes CD37 (TSPAN26) Anticorps
- CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD37 est conjugé à/à la PE
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Application
- Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Attributs du produit
- 4AIPO-24 reacts with CD37 (a.k.a. gp52-40 ), a 40-52 kDa molecule, which is strongly expressed on B cells from the pre-B cell stage, but not on plasma cells. It is also present at low levels on some T cells, monocytes and granulocytes. CD37 is a stable marker for malignancies derived from mature B cells, such as B-CLL, HCL and all types of B-NHL. CD37 is involved in signal transduction.CD37 is stable marker for malignancies derived from mature B cells: B-CLL. HCL and B-NHL.
- Clone
- 4AIPO-24
- Isotype
- IgG2b
- Top Product
- Discover our top product TSPAN26 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))
- Autre désignation
- CD37 (TSPAN26 Produits)
- Synonymes
- anticorps Tspan26, anticorps GP52-40, anticorps TSPAN26, anticorps CD37 antigen, anticorps CD37 molecule, anticorps Cd37, anticorps CD37
- Pathways
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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