LBP anticorps

N° du produit ABIN2191765

Détail du produit anti-LBP anticorps

Antigène: LBP (Lipopolysaccharide Binding Protein (LBP))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LBP est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Blocking Reagent (BR), Immunoassay (IA)

Stérilité: 0.2 μm filtered

Clone: M330-19

Information d'application

Indications d'application: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts LBP to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-LBP (ABIN2191765)

Le Roy, Di Padova, Adachi, Glauser, Calandra, Heumann: "Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2759-65, (2001) (PubMed).

Le Roy, Di Padova, Tees, Lengacher, Landmann, Glauser, Calandra, Heumann et al.: "Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7454-60, (1999) (PubMed).

Détails sur LBP

Antigène: LBP (Lipopolysaccharide Binding Protein (LBP))

Autre désignation: Lipopolysaccharide Binding Protein (LBP Produits)

Synonymes: anticorps BPIFD2, anticorps Bpifd2, anticorps Ly88, anticorps LPSBP, anticorps LBP, anticorps lipopolysaccharide binding protein, anticorps lipopolysaccharide-binding protein, anticorps LBP, anticorps Lbp, anticorps LOC100472839

Sujet: The monoclonal antibody M330-19 reacts highly specific with mouse natural and recombinant LBP. The antibody is a type I antibody blocking the LPS binding to LBP. LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process