C3 anticorps

N° du produit ABIN2191788

Détail du produit anti-C3 anticorps

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp C3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoassay (IA)

Réactivité croisée (Details): Cross reactivity: Mouse C4 : No

Stérilité: 0.2 μm filtered

Clone: 11H9

Isotype: IgG2a

Information d'application

Indications d'application: For Immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive MPL-lpr kidney cells control F: As positive control brain sections of APPQ mice were used and as negative control isotype matched material Negative Wild type kidney cells control

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Références pour anticorps anti-C3 (ABIN2191788)

Zhou, Fonseca, Pisalyaput, Tenner: "Complement C3 and C4 expression in C1q sufficient and deficient mouse models of Alzheimer's disease." dans: Journal of neurochemistry, Vol. 106, Issue 5, pp. 2080-92, (2008) (PubMed).

Détails sur C3

Antigène: C3 (Complement Component 3 (C3))

Abstract: C3 Produits

Synonymes: anticorps AHUS5, anticorps ARMD9, anticorps ASP, anticorps C3a, anticorps C3b, anticorps CPAMD1, anticorps AI255234, anticorps HSE-MSF, anticorps Plp, anticorps C3, anticorps C3-1, anticorps sb:cb26, anticorps si:dkey-76b14.4, anticorps wu:fi34b03, anticorps wu:fj86e11, anticorps C3d, anticorps asp, anticorps cpamd1, anticorps c3, anticorps complement C3, anticorps complement component 3, anticorps complement component c3a, duplicate 1, anticorps complement component 3 L homeolog, anticorps complement component C3, anticorps complement C3-like, anticorps C3, anticorps c3a.1, anticorps c3.L, anticorps LOC443475, anticorps c3, anticorps c3-1, anticorps LOC100060539

Sujet: The monoclonal antibody 11H9 recognizes mouse complement component C3 and its activation products, C3b, iC3b, C3d and C3dg. The complement system is an important part of the humoral response in innate immunity, consisting of three different pathways. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The complement factor C3 consists of an alpha- and a beta-chain. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/mL. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. The monoclonal antibody 11H9 recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg. These activation products are present in acute as well as chronic inflammatory conditions. In chronic inflammatory condition, primarily the C3dg product resides at the place of inflammation (C3c being cleared) which is recognized by antibody 11H9. The chronic processing/activation of C3 is taking place at a lower level, which would reduce detection of the C3 fragments C3b, iC3b, and C3c. Aliases Complement component C3 Immunogen C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling