For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50 Positive Neutrophils isolated from digested infarcts control 1
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Matthijsen, Huugen, Hoebers, de Vries, Peutz-Kootstra, Aratani, Daha, Tervaert, Buurman, Heeringa: "Myeloperoxidase is critically involved in the induction of organ damage after renal ischemia reperfusion." dans: The American journal of pathology, Vol. 171, Issue 6, pp. 1743-52, (2007) (PubMed).
van Leeuwen, Gijbels, Duijvestijn, Smook, van de Gaar, Heeringa, de Winther, Tervaert: "Accumulation of myeloperoxidase-positive neutrophils in atherosclerotic lesions in LDLR-/- mice." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 1, pp. 84-9, (2007) (PubMed).
Huugen, Xiao, van Esch, Falk, Peutz-Kootstra, Buurman, Tervaert, Jennette, Heeringa: "Aggravation of anti-myeloperoxidase antibody-induced glomerulonephritis by bacterial lipopolysaccharide: role of tumor necrosis factor-alpha." dans: The American journal of pathology, Vol. 167, Issue 1, pp. 47-58, (2005) (PubMed).
The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150 kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice. The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA, in frozen tissue sections fixed with acetone and in flow cytometry using a cell permeabilization method. The antibody 8F4 is cross-reactive with rat MPO. Immunogen purified mouse MPO from WEHI-3 cells