Siglec H anticorps

N° du produit ABIN2191824

Détail du produit anti-Siglec H anticorps

Antigène: Siglec H

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Siglec H est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Inhibition Assay (InhA)

Stérilité: 0.2 μm filtered

Clone: 440c

Information d'application

Indications d'application: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of Siglec-H to be inactivated.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-Siglec H (ABIN2191824)

Blasius, Vermi, Krug, Facchetti, Cella, Colonna: "A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha." dans: Blood, Vol. 103, Issue 11, pp. 4201-6, (2004) (PubMed).

Détails sur Siglec H

Antigène: Siglec H

Autre désignation: Siglec-H (Siglec H Produits)

Synonymes: anticorps 6430529G09Rik, anticorps Siglec-H, anticorps sialic acid binding Ig-like lectin H, anticorps Siglech

Sujet: The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.