Ly6C anticorps

N° du produit ABIN2191831

Détail du produit anti-Ly6C anticorps

Antigène: Ly6C (Ly-6C) (Ly-6C)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Ly6C est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Detection (D), Immunoassay (IA)

Stérilité: 0.2 μm filtered

Clone: ER-MP20

Information d'application

Indications d'application: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-Ly6C (ABIN2191831)

Manitz, Horst, Seeliger, Strey, Skryabin, Gunzer, Frings, Schönlau, Roth, Sorg, Nacken: "Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro." dans: Molecular and cellular biology, Vol. 23, Issue 3, pp. 1034-43, (2003) (PubMed).

Henkel, McKercher, Leenen, Maki: "Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1." dans: Blood, Vol. 93, Issue 9, pp. 2849-58, (1999) (PubMed).

Chan, Leenen, Bertoncello, Nishikawa, Hamilton: "Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression." dans: Blood, Vol. 92, Issue 4, pp. 1423-31, (1998) (PubMed).

de Bruijn, Slieker, van der Loo, Voerman, van Ewijk, Leenen: "Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens." dans: European journal of immunology, Vol. 24, Issue 10, pp. 2279-84, (1994) (PubMed).

Détails sur Ly6C

Antigène: Ly6C (Ly-6C) (Ly-6C)

Abstract: Ly-6C Produits

Synonymes: anticorps AA682074, anticorps AA959465, anticorps Ly-6C, anticorps Ly-6C1, anticorps Ly6c, anticorps lymphocyte antigen 6 complex, locus C1, anticorps Ly6c1

Sujet: The monoclonal antibody ER-MP20 specifically reacts with mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes). The antigen is a 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen. The monoclonal antibody ER-MP20 has been raised after immunization of rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells. The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan- macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.