For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Date de péremption
12 months
Leenen, Radosevi?, Voerman, Salomon, van Rooijen, Klatzmann, van Ewijk: "Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2166-73, (1998) (PubMed).
Allaerts, Salomon, Leenen, van Wijngaardt, Jeucken, Ruuls, Klatzmann, Drexhage: "A population of interstitial cells in the anterior pituitary with a hematopoietic origin and a rapid turnover: a relationship with folliculo-stellate cells?" dans: Journal of neuroimmunology, Vol. 78, Issue 1-2, pp. 184-97, (1997) (PubMed).
Leenen, Melis, Kraal, Hoogeveen, Van Ewijk: "The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity." dans: European journal of immunology, Vol. 22, Issue 6, pp. 1567-72, (1992) (PubMed).
The monoclonal antibody ER-BMDM1 is directed against a 160- kDa cell membrane-associated antigen with aminopeptidase N activity. Aminopeptidase N (APN) is also known as Alanyl Aminopeptidase (ANPEP), CD13 antigen or membrane protein p161. The protein consists of a short cyplasmic tail, a transmembrane region, a Ser/Thr region, and a zinc metalloproteinase domain. Widely expressed in many cells, tissues, and species, aminopeptidase N cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. It has putative involvement in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis and neurotransmitter degradation. The antigen recognized by the mocnoclonal antibody ER-BMDM1 is present on various mouse macrophage and dendritic cell subpopulations as well as on microvillous epithelia. The ER-BMDM1 antigen is expressed at increasing levels upon maturation of macrophages. Therefore the monoclonal antibody ER-BMDM1 is a useful marker for the identification of aminopeptidase N positive macrophages and dendritic cells in mouse, the expression of the ER- BMDM1 antigen arises after the monocytic stage of differentation: bone marrow cells and peripheral blood monocytes are ER-BMDM1 negative, whereas mature macrophages express the ER-BMDM1 antigen. A subpopulation of dendrocytes and their in vitro equivalents, the dendritic cells, are also ER- BMDM1 positive.