RBC
Reactivité: Cobaye
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Mouse erythrocytes control
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Baudino, Nimmerjahn, Azeredo da Silveira, Martinez-Soria, Saito, Carroll, Ravetch, Verbeek, Izui: "Differential contribution of three activating IgG Fc receptors (FcgammaRI, FcgammaRIII, and FcgammaRIV) to IgG2a- and IgG2b-induced autoimmune hemolytic anemia in mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 3, pp. 1948-53, (2008) (PubMed).
Fossati-Jimack, Azeredo da Silveira, Moll, Kina, Kuypers, Oldenborg, Reininger, Izui: "Selective increase of autoimmune epitope expression on aged erythrocytes in mice: implications in anti-erythrocyte autoimmune responses." dans: Journal of autoimmunity, Vol. 18, Issue 1, pp. 17-25, (2002) (PubMed).
Azeredo da Silveira, Kikuchi, Fossati-Jimack, Moll, Saito, Verbeek, Botto, Walport, Carroll, Izui: "Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high affinity anti-erythrocyte autoantibody." dans: The Journal of experimental medicine, Vol. 195, Issue 6, pp. 665-72, (2002) (PubMed).
Shibata, Berney, Reininger, Chicheportiche, Ozaki, Shirai, Izui: "Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms." dans: International immunology, Vol. 2, Issue 12, pp. 1133-41, (1991) (PubMed).
The monoclonal antibody 34-3C recognizes an exposed surface determinant of intact red blood cells (RBC). The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity. The monoclonal antibody only recognises antigenic determinants expressed on Mouse RBC and not on other species of RBC. Immunogen RBC