For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
van der Laan, de Leij, Buurman, Timens, ten Duis: "Tumor necrosis factor alpha (TNFalpha) in human skin: a comparison of different antibodies for immunohistochemistry." dans: Archives of dermatological research, Vol. 293, Issue 5, pp. 226-32, (2001) (PubMed).
Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." dans: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, (2000) (PubMed).
Limb, Chignell, Green, LeRoy, Dumonde: "Distribution of TNF alpha and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy." dans: The British journal of ophthalmology, Vol. 80, Issue 2, pp. 168-73, (1996) (PubMed).
The antibody reacts with human native and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant TNF-alpha as determined with L929 cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF-alpha and lacks crossreactivity with human lymphotoxin.