For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Heart cells, recombinant human H-FABP control 1
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Zhen, Berna, Jin, Pritt, Watson, Ackermann, Hale: "Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necroses." dans: Proteomics. Clinical applications, Vol. 1, Issue 7, pp. 661-71, (2010) (PubMed).
Pelsers, Hanhoff, Van der Voort, Arts, Peters, Ponds, Honig, Rudzinski, Spener, de Kruijk, Twijnstra, Hermens, Menheere, Glatz: "Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility." dans: Clinical chemistry, Vol. 50, Issue 9, pp. 1568-75, (2004) (PubMed).
Roos, Eymann, Symannek, Duppenthaler, Wodzig, Pelsers, Glatz: "Monoclonal antibodies to human heart fatty acid-binding protein." dans: Journal of immunological methods, Vol. 183, Issue 1, pp. 149-53, (1995) (PubMed).
Antigène
FABP3
(Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))
The monoclonal antibody 67D3 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. Furthermore, this antibody is useful for the purification of H-FABP. Aliases Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor Immunogen Purified human H-FABP