Lipid A anticorps

N° du produit ABIN2191914

Détail du produit

Antigène: Lipid A

Reactivité: Chemical

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

niveau d'endotoxine: Low endotoxin level

Clone: 43

Information d'application

Indications d'application: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Stockage

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Référence

Chou, Chuang, Eng, Chen, Wang, Chen, Lin: "Endotoxin and CD14 in the progression of biliary atresia." dans: Journal of translational medicine, Vol. 8, pp. 138, (2011) (PubMed).

Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "The antibody reactivity of monoclonal lipid A antibodies is influenced by the acylation pattern of lipid A and the assay system employed." dans: Immunobiology, Vol. 189, Issue 5, pp. 457-71, (1994) (PubMed).

Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A." dans: Infection and immunity, Vol. 60, Issue 6, pp. 2201-10, (1992) (PubMed).

Détail du antigène

Antigène: Lipid A

Classe de substances: Chemical

Sujet: The monoclonal antibody 43 reacts with the hydrophilic backbone of free lipid A. This antibody only reacts with lipid A not covalent bound to LPS. LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consists of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.