MADCAM1 anticorps (FITC)

N° du produit ABIN2192056

Détail du produit anti-MADCAM1 anticorps (FITC)

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MADCAM1 est conjugé à/à la FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA), Inhibition Assay (InhA)

Stérilité: 0.2 μm filtered

Clone: 314G8

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of MAdCAM-1 to be inactivated.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-MADCAM1 (FITC) (ABIN2192056)

Leung, Lehnert, Kanwar, Yang, Mon, McNeil, Krissansen: "Bioassay detects soluble MAdCAM-1 in body fluids." dans: Immunology and cell biology, Vol. 82, Issue 4, pp. 400-9, (2004) (PubMed).

Détails sur MADCAM1

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Autre désignation: Madcam-1 (MADCAM1 Produits)

Synonymes: anticorps MACAM1, anticorps AV211525, anticorps mucosal vascular addressin cell adhesion molecule 1, anticorps MADCAM1, anticorps Madcam1

Sujet: The monocolonal antibody 314G8 reacts with human mucosal addressin cell adhesion molecules-1 (MAdCAM-1), a key player in mediating the infiltration of leukocytes into chronically inflamed tissue. MAdCAM-1 is a cell-surface Ig superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain and a short cytoplasmatic domain. It interacts via its N- terminal Ig domain with the lymphocyte homing receptor alpha4beta7, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T- and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. Mucosal addressin cell adhesion molecule-1 RNA transcripts are predominantly expressed in the small intestine, mesenteric lymph nodes, colon and spleen, and are very weakly expresssed in human pancreas and brain. The monocolonal antibody 314G8 recognizes a site in the N- terminal Ig domain of MAdCAM-1. The monoclonal antibody 314G8 detects MAdCAM-1 on venules in the spleen and small intestine. MAdCAM-1 is strongly expressed in the synovium of osteoarthritis patients, predominantly on the endothelial lining of blood vessels, but also within the vessel lumen. The monoclonal antibody 314G8 may be useful in diagnosis of inflammation in humans by monitoring the presence and levels of MAdCAM-1.