MARCO anticorps

N° du produit ABIN2192057

Détail du produit anti-MARCO anticorps

Antigène: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MARCO est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Réactivité croisée (Details): Cross reactivity: Bovine : Yes, Chicken : No

Stérilité: 0.2 μm filtered

Clone: PLK-1

Isotype: IgG3

Information d'application

Indications d'application: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Human alveolar macrophages control Negative All other human cells control

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Références pour anticorps anti-MARCO (ABIN2192057)

Arredouani, Palecanda, Koziel, Huang, Imrich, Sulahian, Ning, Yang, Pikkarainen, Sankala, Vargas, Takeya, Tryggvason, Kobzik: "MARCO is the major binding receptor for unopsonized particles and bacteria on human alveolar macrophages." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6058-64, (2005) (PubMed).

Bunn, Kobzik, Grigg: "MARCO expression on pediatric alveolar macrophages." dans: Cytometry. Part B, Clinical cytometry, Vol. 60, Issue 1, pp. 54-6, (2004) (PubMed).

Palecanda, Kobzik: "Receptors for unopsonized particles: the role of alveolar macrophage scavenger receptors." dans: Current molecular medicine, Vol. 1, Issue 5, pp. 589-95, (2002) (PubMed).

Détails sur MARCO

Antigène: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Autre désignation: Marco (MARCO Produits)

Synonymes: anticorps AI323439, anticorps Ly112, anticorps Scara2, anticorps SCARA2, anticorps macrophage receptor with collagenous structure, anticorps MARCO, anticorps Marco

Sujet: The monoclonal antibody PLK-1 recognizes the macrophage receptor with collagenous structure (MARCO). The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although it's expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding. Aliases SCARA2, scavenger receptor class A member 2, macrophage receptor with collagenous structure Immunogen Human alveolar macrophages (recognizes domain V between residues 420 and 431)

Pathways: Activation of Innate immune Response