Nitrotyrosine anticorps

N° du produit ABIN2192139

Détail du produit anti-Nitrotyrosine anticorps

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA)

Réactivité croisée (Details): Cross reactivity: Phosphotyrosine : No, Chlorotyrosine : No

Stérilité: 0.2 μm filtered

Clone: HM-11

Isotype: IgG2b

Information d'application

Indications d'application: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive W: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate control P: human lung tissue

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Références pour anticorps anti-Nitrotyrosine (ABIN2192139)

Tsuhako, Augusto, Linares, Chadi, Giorgio, Pereira: "Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation." dans: Free radical biology & medicine, Vol. 48, Issue 5, pp. 704-12, (2010) (PubMed).

Han, Drabek, Stezoski, Janesko-Feldman, Stezoski, Clark, Bayir, Tisherman, Kochanek: "Protein nitration and poly-ADP-ribosylation in brain after rapid exsanguination cardiac arrest in a rat model of emergency preservation and resuscitation." dans: Resuscitation, Vol. 79, Issue 2, pp. 301-10, (2008) (PubMed).

Casoni, Basso, Massignan, Gianazza, Cheroni, Salmona, Bendotti, Bonetto: "Protein nitration in a mouse model of familial amyotrophic lateral sclerosis: possible multifunctional role in the pathogenesis." dans: The Journal of biological chemistry, Vol. 280, Issue 16, pp. 16295-304, (2005) (PubMed).

Klausz, Tiszai, Tiszlavicz, Gyulai, Lénárt, Lonovics, Mándi: "Local and peripheral cytokine response and CagA status of Helicobacter pylori-positive patients with duodenal ulcer." dans: European cytokine network, Vol. 14, Issue 3, pp. 143-8, (2003) (PubMed).

ter Steege, Koster-Kamphuis, van Straaten, Forget, Buurman: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." dans: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Abstract: Nitrotyrosine Produits

Classe de substances: Chemical

Sujet: The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage. The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. Immunogen Nitrated KLH