Cet anticorps anti-MHV NSP9 Monoclonal Souris (Clone 2C6-H1) (ABIN233751) détecte spécifiquement MHV NSP9 dans WB et FM.
L’anticorps est réactif avec des échantillons de Mouse Hepatitis Virus (MHV).
Aperçu rapide pour MHV NSP9 anticorps (ABIN233751)
Antigène
MHV NSP9
Reactivité
Mouse Hepatitis Virus (MHV)
Hôte
Souris
Clonalité
Monoclonal
Conjugué
Inconjugué
Application
Western Blotting (WB), Fluorescence Microscopy (FM)
Clone
2C6-H1
N° du produit (Fournisseur)
200-301-a56
Fournisseur
Rockland
Attributs du produit
Concentration Definition: by UV absorbance at 280 nm
Immunogène
This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein. Immunogentype:Native
Reactivité: SARS Coronavirus-2 (SARS-CoV-2)
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
Restrictions
For Research Use only
Format
Liquid
Concentration
2.1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Antigène
MHV NSP9
Classe de substances
Viral Protein
Sujet
The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes. Synonyms: MHV-A59 nsp9 protein antibody