Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Réactivité croisée
Souris, Chimpanzé, Rat (Rattus)
Attributs du produit
Concentration Definition: by UV absorbance at 280 nm
Immunogène
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an of human Myosin 1G protein.
MYO1G
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
FITC
Indications d'application
This affinity-purified antibody has been tested for use in ELISA, western blotting and possibly in immunoprecipitation as well. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 100 kDa in size corresponding to Myosin 1G protein is expected in the appropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.2 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Olety, Wälte, Honnert, Schillers, Bähler: "Myosin 1G (Myo1G) is a haematopoietic specific myosin that localises to the plasma membrane and regulates cell elasticity." dans: FEBS letters, Vol. 584, Issue 3, pp. 493-9, (2010) (PubMed).
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. In general, myosins are protein complexes consisting of one or more myosin heavy chains, associated light chains and other proteins. Myosins function as molecular motors and use the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments. Myosins have magnesium-ATPase activity and bind actin. Myosins can be divided into classes that are distinguished based on sequence features of the motor, or head domain, but also have distinct tail regions that are believed to bind specific cargoes. Unconventional myosins exist. Myosin 1G is an unconventional myosin that is restricted to hematopoietic cells. Unconventional myosins are also critical for motility in amoeba and a mammalian paralog (Myo1C) is critical as a glucose transporter that recycles glucose in response to insulin. Synonyms: Myosin-Ig Cleaved into the following chain: 1.Minor histocompatibility antigen HA-2 mHag HA-2