Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Réactivité croisée
Souris, Rat (Rattus)
Attributs du produit
Concentration Definition: by UV absorbance at 280 nm
Immunogène
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. This antibody reacts strongly with the acetylated form of the immunizing peptide and shows minimal reactivity with the non-acetylated form when tested by ELISA. To date, western blotting shows equivalent staining of lysates either treated or untreated with Trichostatin A (an HDAC inhibitor). Therefore, western blotting results are not definitive for demonstrating the specificity of this reagent. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
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Deskin, Lasky, Zhuang, Shan: "Requirement of HDAC6 for activation of Notch1 by TGF-β1." dans: Scientific reports, Vol. 6, pp. 31086, (2018) (PubMed).
Mo, Zhao, Liu, Hu, Chen, Pham, Wei, Liu, Zhou, Burgess, Pfaff, Caskey, Wu, Bai, Yang: "Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy." dans: Nature communications, Vol. 9, Issue 1, pp. 1007, (2018) (PubMed).
Dedes, Dedes, Imesch, von Bueren, Fink, Fedier: "Acquired vorinostat resistance shows partial cross-resistance to 'second-generation' HDAC inhibitors and correlates with loss of histone acetylation and apoptosis but not with altered HDAC and HAT activities." dans: Anti-cancer drugs, Vol. 20, Issue 5, pp. 321-33, (2009) (PubMed).
anticorps EL52, anticorps HSP86, anticorps HSP89A, anticorps HSP90A, anticorps HSP90N, anticorps HSPC1, anticorps HSPCA, anticorps HSPCAL1, anticorps HSPCAL4, anticorps HSPN, anticorps Hsp89, anticorps Hsp90, anticorps LAP2, anticorps git10, anticorps swo1, anticorps HSP90, anticorps htpG, anticorps SCBAC25F8.08, anticorps 23.m06066, anticorps 17.m07646, anticorps HSP90-1, anticorps 143198_at, anticorps 83, anticorps 83K HSP, anticorps DMHSP82, anticorps E(sev)3A, anticorps E(sina)2, anticorps HSP82, anticorps HSP83, anticorps ORF1, anticorps Su(Raf)3A, anticorps anon-EST:Liang-2.53, anticorps anon-WO0068693, anticorps anon-WO0140519.209, anticorps clone 2.53, anticorps en(lz)3C/4C, anticorps hsp84, anticorps l(3)j5C2, anticorps ms(3)08445, anticorps stc, anticorps DmelCG1242, anticorps CG1242, anticorps Hsp86, anticorps Hspca, anticorps 86kDa, anticorps 89kDa, anticorps AL024080, anticorps AL024147, anticorps Hsp86-1, anticorps hsp4, anticorps hsp86, anticorps hsp89, anticorps hsp90, anticorps hsp90a, anticorps hspc1, anticorps hspca, anticorps hspn, anticorps lap2, anticorps Hsp90alpha, anticorps heat shock protein 90 alpha family class A member 1, anticorps heat shock protein Hsp90, anticorps Hsp90 chaperone, anticorps Heat shock protein 90, anticorps heat shock protein 90, anticorps chaperone protein HtpG, anticorps molecular chaperone HtpG, anticorps Heat Shock Protein 90, anticorps uncharacterized LOC100384473, anticorps Heat shock protein 83, anticorps heat shock protein 90, alpha (cytosolic), class A member 1, anticorps heat shock protein 90 alpha family class B member 1, anticorps heat shock protein 90kDa alpha family class A member 1 L homeolog, anticorps heat shock protein HSP 90-alpha, anticorps HSP90AA1, anticorps hsp90, anticorps HSP90, anticorps daf-21, anticorps htpG, anticorps SCO7516, anticorps MAP_RS10510, anticorps TP04_0646, anticorps TP01_0934, anticorps APH_RS03525, anticorps GbCGDNIH1_0315, anticorps MAV_2118, anticorps HSP90C, anticorps BBOV_IV008400, anticorps BBOV_III007380, anticorps ACICU_00312, anticorps ECL_01244, anticorps YE105_C1172, anticorps pco153543(105), anticorps Hsp83, anticorps Hsp90aa1, anticorps HSP90AB1, anticorps hsp90aa1.1.L, anticorps LOC108698781
Sujet
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Hsp90 is a member of the heat shock protein 90 family, the members of which are highly conserved between isoforms and species. Hsp90 functions as a molecular chaperone and has ATPase activity. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo and interacts with TOM34, AHSA1, HDAC6 and SMYD3. Several signal transduction pathways depend on Hsp90 function, including pathways involving erbB2, hypoxia sensitivity (Hif1 alpha), and steroid hormone receptors (for example, androgen, progesterone, glucocorticoid, and aryl-hydrocarbon). Recent reports show that Hsp90 from tumor cells has increased sensitivity to small molecule inhibitors (for example, 17AAG). The mechanism of the differential sensitivity of Hsp90 from normal versus tumor cells is unknown, although mutation has been ruled out. One possible mechanism may be differences in post-translational modification of tumor Hsp90. K294 was found to be acetylated in purified Hsp90 from SkBr3 cells, a breast cancer cell line. Synonyms: Heat shock protein HSP 90-alpha Gene names Name: HSP90AA1 Synonyms: HSP90A, HSPCA